Single Nucleotide Polymorphisms Can Create Alternative Polyadenylation Signals and Affect Gene Expression through Loss of MicroRNA-Regulation
Figure 2
Panels (A) and (B) show 3′ ends of the MIER1 and PNN genes as annotated in PolyA_Db (3′ ends of the horizontal lines), and their candidate APA SNP. The four other graphs show the inverse cumulative distribution of EST sequence ending position for APA alleles (triangles) and non-APA alleles (circles). The dashed vertical line shows the threshold separating short and long transcripts. The transcript proportion is decreasing before the threshold for APA alleles, compared to non-APA alleles. This decrease indicates that APA alleles are more likely to produce shorter transcripts. Panels (A), (C) and (E) show the MIER1 gene. Panels (B), (D) and (F) show the PNN gene. Several unknown alleles could be imputed through haplotypes (included in Panels (C) and (D)).