Modular Design of Artificial Tissue Homeostasis: Robust Control through Synthetic Cellular Heterogeneity
Figure 8
Phenotypic sensitivity analysis.
(A,F) Phenotypic behavior of the oscillator (A) and throttle (F), when isolated from the full system. Roughly 2000 different sets of rate constants were tested, with all oscillator or throttle rate constants simultaneously varied. Module phenotypes were recorded for each set of rate constants. (B) Observed S/N values as a function of variance in the “duration high” of the oscillator. (C) Heat map of the S/N values against the phenotypes resulting from the random parameter sets. (G) Average ‘images’ for the phenotype R7 T to St. St., observed from the random parameter sets yielding an S/N value of either 5, 15 or 25. Black represents regions where no switch occurs and no value for R7 T to St. St. is recorded. (D,H) The most significant RS-HDMR sensitivity indices, , for phenotypic variations of the oscillator and throttle, respectively (see also Supplementary Table S8). (E,I) For the oscillator and throttle, respectively, RS-HDMR cross-validation predication accuracy using rate constants, phenotypes, or both.