Emergence of a Small-World Functional Network in Cultured Neurons
Figure 5
Change in the link lengths with culture development.
Panel A: Each bar represents the median proportion of links between nodes up to (and including) two electrodes apart (classified as ‘nearby’) and links between nodes greater than two electrodes apart (classified as ‘distant’), diagonal neighbors were included; values were calculated from all cultures at each age. Upwards error bars represent the 75th percentile and downwards bars the 25th percentile. Notably, at DIV 14 there was a significantly higher number of connections between distant nodes. Panel B: Normalized histograms of link lengths at each culture age, constructed from the link lengths of all cultures, measured as the proportion of each culture's links at each length. Median values from all cultures were used for each bin in the histogram. Bin size was based on spacing between electrodes of MEA, with one bin for each electrode distance (i.e. bin 1 is all links between neighboring electrodes - including diagonal neighbors, bin 2 is all links between nodes up to two electrodes distance, and so forth until seven electrodes distance which is the maximum between any two nodes on the MEA). Bin edges (X-axis) specify the start of each bin, measured as the distance between electrodes on the MEA (micrometers). Y-axis is the same for all histograms in panel, only DIV 14 Y-axis is labeled to avoid overcrowding.