Comprehensive Modelling of the Neurospora Circadian Clock and Its Temperature Compensation
Figure 8
Clock components levels at 21 and 28°C.
(A) Northern blot analysis of clock-specific transcript levels in strains grown in DD at either 21°C (left panel) or 28°C (right panel). Mycelial discs from wild-type or wc1-myc were grown in liquid culture at 21 or 28°C and tissue harvested at four-hour intervals in the first day of DD. Ethidium bromide stained gels were used to correct for loading. (B) The effects of temperature on clock-specific protein levels. Western blot analysis of clock-specific protein levels in strains grown in DD at either 21°C (left panel), 25°C (representative blot not shown) or 28°C (right panel). Amido black stained membranes were used to correct for loading. (C) Quantitative analysis of Northern blot data shown in A. Maximum transcript levels were set to 100%. (D) Quantitative analysis of western blot data shown in B. Maximum protein levels were set to 100%. The graphs in (C) and (D) represent three independent experiments. Error bars indicate ±1 standard error.