Highly Sensitive and Specific Detection of Rare Variants in Mixed Viral Populations from Massively Parallel Sequence Data
Figure 7
Low frequency variants overwhelmingly called with phase.
Histogram shows low frequency variants overwhelmingly called with phase thresholds. Variants frequencies are estimated by the frequencies of variants among the reads at that position. Versions of V-Phaser with and without phase thresholds called variants on a clinical sample that are binned by their frequency at their locus. Most variants <5% were detected only be V-Phaser with phase thresholds, and the version without phase thresholds detected no variants <1%.