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Highly Sensitive and Specific Detection of Rare Variants in Mixed Viral Populations from Massively Parallel Sequence Data

Figure 2

Phase distance approached length of average read as coverage increased.

The phase distance was longer than half the average read length for loci covered more than 65-fold, and as coverage increased, it approached the length of the average read. The phase distance is a measure of how far apart phased variants can be and still be detected at lower frequencies than variants not in phase. We show the phase distance as a percentage of average read length.

Figure 2

doi: https://doi.org/10.1371/journal.pcbi.1002417.g002