Integrating Flux Balance Analysis into Kinetic Models to Decipher the Dynamic Metabolism of Shewanella oneidensis MR-1
Figure 5
Experimentally observed and simulated isotopomer labeling patterns [M-57]+ in proteinogenic amino acids.
The standard error for GC-MS measurement was below 0.02. A1: dynamic isotopomer simulation for glutamate from dFBA without considering reaction reversibility (dFBA w/o reversibility). A2: dynamic isotopomer simulation for glutamate from dFBA considering reaction reversibility (dFBA w/ reversibility). Bar plot: comparison of experimentally observed isotopomer labeling to simulated isotopomer labeling patterns of glutamate (A1: without considering reaction reversibility; A2: considering reaction reversibility). B: The model fitting of the isotopomer labeling data of five key amino acids (Ala, Gly, Ser, Asp, and Glu) at t = 24 and 30 h.