Multi-Scale Modeling of HIV Infection in vitro and APOBEC3G-Based Anti-Retroviral Therapy
Figure 8
Distribution of cell states during the period of post infection.
We simulated cultures of 500,000 healthy normal cells inoculated by either (A) 10 ng p24 or (B) 1 ng p24 WT HIV input. The infected cells start producing new virions after 22 hours and eventually die around 55 hours after infection. For WT HIV input, most of the cells are dead by the 12th day. In contrast, if we inoculate the cultures with either (C) 10 ng p24 or (D) 1 ng p24 ΔVif viruses, the healthy cells will still be the majority ones and the number of dead cells is negligible on the 15th day. In a different scenario, we included effects of cell culture crowding in our multicellular model by using a logistic function. Such cultures inoculated with either (E) 10 ng p24 or (F) 1 ng p24 ΔVif viruses provide better fits to biological experiments.