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Evidence for Sequential and Increasing Activation of Replication Origins along Replication Timing Gradients in the Human Genome

Figure 10

Analysis of replication bubble and λ-SNS coverage in ENCODE CTRs and TTRs.

Replication bubble data are from log-phase HeLa Rep4 library [62] and λ-SNS data are from [64]. (A) Replication bubble coverage is plotted against apparent replication speed of CTRs (blue circles) and TTRs (dark circles). (B) An exemplary ENCODE region. Replication timing profile (dark wavy line) of region ENm001 (grey line) and its surroundings with replication bubbles (cyan) and λ-SNS (orange) in CTRs (red) and TTRs (green) are shown. (C) Replication bubble coverage computed by 100 kb adjacent windows along ENCODE regions is plotted against replication time. (D) λ-SNS coverage is plotted against apparent replication speed of CTRs (blue circles) and TTRs (dark circles). (E) λ-SNS coverage computed by 100 kb adjacent windows along ENCODE regions is plotted against replication time.

Figure 10

doi: https://doi.org/10.1371/journal.pcbi.1002322.g010