Computational Mass Spectrometry–Based Proteomics
Figure 4
Analysis of the acquired spectra includes (a, b) signal processing, (c, d) significance analysis, and (e–h) downstream analysis. Methods in (a–d) must reflect the technological properties of the workflows. Methods in (e–h) are technology-independent and are similar to the analysis of gene expression microarrays, but their use is affected by uncertainty in protein identities and the incomplete sampling of the proteome.