Computational Modeling and Analysis of Insulin Induced Eukaryotic Translation Initiation
Figure 4
Blind model predictions for the ensemble (N = 400).
The predictive ability of model ensemble was assessed by comparing model performance with novel experimental data. Dotted lines represent the simulation mean of the ensemble, while the shaded region denotes the 99.9% confidence estimate for the mean. The solid dots represent the scaled experimental data. A. In vitro time course for formation of 43S-mRNA complex. A slowly-hydrolyzable GTP homologue (GMP-PNP) was used in place of GTP to isolate formation of this intermediate complex. GMP-PNP data was used for training while GTP data was used for validation. B. Percent of Rheb-GTP to Rheb-GDP in the presence of insulin, wortmannin and rapamycin (A14 NIH 3T3 cells). C. Percent of Rheb-GTP to Rheb-GDP in wildtype and TSC2 lacking cells (MEF cells). D. 4EBP1 bound EIF4E in the presence of heat shock (CHO.K1 cells).