Computational Modeling and Analysis of Insulin Induced Eukaryotic Translation Initiation
Figure 3
Ensemble performance against selected training objectives (N = 400).
Dotted lines represent the simulation mean of the ensemble, while the shaded region denotes the 99.9% confidence estimate for the mean. The solid dots represent the scaled experimental data. A. Time course data for p70S6K1 phosphorylation in response to insulin stimulation (L6 Myotubes). B. Time course data for c4EBP1 phosphorylation in response to FBS (RhoE 3T3 cells). C. In vitro time course of the 80S complex measured by puromycin assay (rabbit reticulocyte). D. pAkt(Ser473) levels at 20 minutes in the presence and absence of insulin and wortmannin (393T cells). E,F. pAkt(Set473) and activated p70S6K1 levels at 15 minutes in the presence and absence of insulin-like growth factor (IGF) and rapamycin (C2C12 myotubes).