Modelling Reveals Kinetic Advantages of Co-Transcriptional Splicing
Figure 3
The 3′ cleavage and polyadenylation of Ribo1 measured by RT-qPCR.
(A) Schematic of the Ribo1 gene. The positions of the alternative cDNA primers for uncleaved and cleaved/polyadenylated transcripts are shown by the C2 and oligo (dT) arrows respectively. (B) Points indicate uncleaved pre-mRNA (U-pre-mRNA), polyadenylated pre-mRNA (P-pre-mRNA) and polyadenylated lariat-exon2 (P-lariat-exon2) data. (C) Points indicate 3′ uncleaved lariat-exon2 (U-lariat-exon2), uncleaved mRNA (U-mRNA) and polyadenylated mRNA (P-mRNA) data. Error bars show the standard error of three biological replicates. Solid lines are model predictions.