Modelling Reveals Kinetic Advantages of Co-Transcriptional Splicing
Figure 2
The Ribo1 reporter and its response to doxycycline.
(A) Schematic of the promoter, intron and exons of Ribo1. The positions of the RT-qPCR primers are shown by the arrow C1. (B) Induction and splicing of Ribo1 measured by RT-qPCR. (C) Induction and step 1 splicing of 3′SSRibo1. Step two of splicing is blocked and therefore mRNA is not produced. (D) Induction of 5′SSRibo1. Step one of splicing is blocked and therefore neither lariat-exon2 nor mRNA is produced. Points indicate pre-mRNA (blue), lariat-exon2 (green) and mRNA (black) data. Error bars show the standard error of three biological replicates. Solid lines are model predictions.