Computation of Steady-State Probability Distributions in Stochastic Models of Cellular Networks
Figure 6
(A) T7 RNAP enhances its own transcription. In addition, T7 RNAP expression slows down cell growth, which dilutes T7 RNAP. (B) Probability distribution with M = 10, k0 = 0.001, kf = 0.01, kb = 0.1, k1 = 0.01, dx0 = 0.003, μ = 0.01, and θ = 1; shown with (green) and without (blue) extrinsic noise (σ = 3). (C) Comparison of experiment and modeling on the perturbations to growth and T7 RNAP synthesis rates. Contours denote computed fractions of ON cells, by varying parameters k1 and μ. X's denote experimental data points with experimental parameters (IPTG concentration and OD) as labeled; the k1 and μ values for each data point were determined by fitting the fractions of ON cells.