Construction and Analysis of an Integrated Regulatory Network Derived from High-Throughput Sequencing Data
Figure 1
Schematic diagram of the construction and analysis of the integrative regulatory network.
ChIP-seq data were used to determine target genes and miRNAs of transcription factors. miRNA target genes were predicted using PicTar or TargetScan algorithms together with conservation information. The three types of regulations form the basic network. The sign of each regulatory interaction was determined based on the correlation between TF binding and gene expression, Extra edges of protein-protein or TF-TF combinatorial interactions were incorporated. We studied the topological structure of the integrated network, including hierarchical organization and motif enrichment.