MCAM: Multiple Clustering Analysis Methodology for Deriving Hypotheses and Insights from High-Throughput Proteomic Datasets
Figure 5
Dynamics for SHC phosphorylations and their robust co-clustered partners in EGF treatment of parental and HER2 overexpressing (24H) cells.
For these plots, all subsets were normalized to their own 5 minute time point to make the comparisons across treatments easier. Peptide centric clusters were created by finding all peptides that co-cluster with a given peptide at least 50% of the time in . A) Both SHC1 Y349 and SHC1 Y427 centric clusters are the same for Parental HMEC cells treated with EGF. B) EGF treatment of 24H cells creates some change in the SHC1 Y427 centric cluster, but the dynamics compared to Parental treatment are relatively similar. C) The SHC1 Y349 centric cluster has changed drastically compared to Parental EGF treatment due to a very different response of Y349 phosphorylation.