Colocalization of Protein Kinase A with Adenylyl Cyclase Enhances Protein Kinase A Activity during Induction of Long-Lasting Long-Term-Potentiation
Figure 9
Spatial specificity of cAMP, but not PKA activity.
(A) Concentration of cAMP (A1) and calcium (A2) in spines. Traces for the stimulated spines are toward the back; not all spines are illustrated. The concentration is highest in the stimulated and adjacent spines. (B) Concentration of cAMP and phospho-inhibitor versus time and space. The y-axis has been flipped, with 0 µm at the top to enhance correspondence with (A). Concentration is indicated by scale bar on the right. (B1) shows that cAMP exhibits spatial specificity. Arrows from traces in A1 to y-axis of B1 demonstrates spatial correspondence. (B2) shows that phospho-inhibitor-1 does not exhibit spatial specificity at this scale. (C) Time slices shortly after the stimulation at 10 s, 90 s and 170 s plotted against distance from the stimulated end of the dendrite. (C1) cAMP concentration exhibits spatial specificity, and does not increase with subsequent trains. (C2) Phospho-inhibitor-1 increases with subsequent trains, but does not exhibit spatial specificity.