Colocalization of Protein Kinase A with Adenylyl Cyclase Enhances Protein Kinase A Activity during Induction of Long-Lasting Long-Term-Potentiation
Figure 4
PKA activity is higher when PKA is colocalized with adenylyl cyclase (AC).
(A) With AC in the spine, PKA activity is greater when PKA is anchored in the spine (red trace), than when PKA is anchored in the dendrite (pink trace). (B) With AC located in the dendrite (green and blue traces), the effect of PKA anchoring is not apparent. (C) Effect of colocalization is more apparent when averaging over five trials. The quantity of free PKA catalytic subunit is greater when PKA is colocalized with AC in the dendrite (green trace) than when PKA is separated from the AC (blue and pink traces). (D) Total PKA activity (calculated as area under the curve describing concentration of the free catalytic subunit of PKA), reveals that colocalization of AC and PKA produces significantly greater PKA activity than when AC and PKA are anchored apart.