Identification of Hammerhead Ribozymes in All Domains of Life Reveals Novel Structural Variations
Figure 6
Variant hammerhead ribozymes encoded in saltern-derived DNA.
(A) Secondary structure models of variants HHmeta and HHphage. Annotations are as described for Figures 2 and 5. Residues corresponding to the highly atypical insertions are numbered 2a and 2b. Guanosine residues depicted in lowercase were added to facilitate transcription in vitro. (B) Effect of MgCl2 concentration on the kobs of HHmeta. kobs values were determined in the absence of KCl (open circles) or in the presence of 3 M KCl (filled circles).