MDCK Cystogenesis Driven by Cell Stabilization within Computational Analogues
Figure 2
Quantitative measures of in vitro and in silico cystogenesis.
Mean values and standard deviations for (A) cell number per cyst, (B) cyst and lumen area, (C) mean individual cell area and (D) ratio: cellular to cyst area. Blue: in vitro data taken each day for ten days from 20 cysts. Red: data taken from 50 cysts over ten days using the parameter values in Table 2. Gray boxes: noted changes in behavior. Blue lines: slope of in vitro growth illustrating changes in rate. SSM1: Self -Similarity Measure of in vitro growth; SSM1 indicates the percentage of in vitro values each day that fell within ±25% of the mean in vitro value for that day. SM1: Similarity Measure for ISMA growth. SM1 indicates the percentage of ISMA values each day that fell within ±25% of the mean in vitro value for that day. The target was that SM1>0.5 for nine of ten days. When the target was met, we posited that ISMA measures were experimentally indistinguishable from in vitro measures. Gray SM values did not achieve targeted values.