“Fluctuograms” Reveal the Intermittent Intra-Protein Communication in Subtilisin Carlsberg and Correlate Mechanical Coupling with Co-Evolution

doi:10.1371/journal.pcbi.1002023

“Fluctuograms” Reveal the Intermittent Intra-Protein Communication in Subtilisin Carlsberg and Correlate Mechanical Coupling with Co-Evolution

Figure 8

Correlating the fluctuograms of EG1 with co-evolution.

(a) The highly correlated residues observed in a multiple sequence alignment and SCA using EG1 as the query sequence and the residues selected from the fluctuogram satisfying either of the three criteria with the following parameters: = 8, = 2.5, = 17, = 8.0, and = 0.8. Lime: residues that cover the co-evolved residues from SCA. Brown: co-evolved residues from SCA that are not covered by the residues selected from the fluctuogram. Pink: residues selected from the fluctuogram but do not cover any co-evolved residue. (b) the same as (a) but view from a different angle. The calculated hit rates and coverages from the fluctuogram of EG1 by using (c) Criterion-A, (eq.(2)), (d) Criterion-B, (eq.(3)), and (e) Criterion-C, (eq.(4)).

doi: https://doi.org/10.1371/journal.pcbi.1002023.g008