Dynamic Conformational Changes in MUNC18 Prevent Syntaxin Binding
Figure 5
The closure of the cavity as detected by ED analyses of munc18aS306D, S313D simulation.
A) Porcupine plot, presenting the first eigenvector in the mutant munc18aS306D,S313D simulation as produced by ED analysis (M1) performed by the Dynatraj tool. B) Comparison of the magnitudes of the eight main eigenvectors of the GROMACS-based ED analysis extracted from the simulations of wild-type munc18a (simulation 1, blue squares) and munc18aS306D,S313D (simulation M1, green squares). C, D) Snapshots of the first (t = 0, C) and the last (t = 35 ns, D) frames of the second munc18aS306D,S313D (Simulation M2, Table 1) simulation.