Digital Atlasing and Standardization in the Mouse Brain
Figure 5
The Edinburgh Mouse Atlas Project.
To link EMAP with WHS, the Waxholm volume was transformed into EMAP's native representation (b) and then mapped into EMAP Theiler Stage 23 (TS23) space, the result shown in (a). Similarly, the result of mapping the EMAP model into WHS is shown in (c). A few registration landmarks are shown in (a, b) to illustrate the process. The final transformation was established by anatomists who aligned recognizable tissue boundaries to within about five voxels in WWHS (∼100 microns). A prototype for an Edinburgh INCF hub allows access to EMAP and the related EMAGE gene expression databases available through the INCF-DAI (d). In addition to image processing–based mappings, alternative methods of mappings are being explored, including ontology-based mappings, and mapping of areas of interest across atlases using spatial rules (d).