Digital Atlasing and Standardization in the Mouse Brain
Figure 2
Isotropic 21.5-µm3 resolution MR volumes were generated along with a complete 21-µm Nissl series of the same brain. The WHS origin is defined by the junction of the rostral and dorsal tangential planes of the anterior commissure with the mid-sagittal plane. (a) Nissl histology, (b) T1 image, (c) T2*, this image is used to provide the Nissl-MRI registration (d) fractional anisotropy image, essentially showing white matter. Several cytoarchitectonic domains can be identified at this resolution, including individual cortical layers within allocortex and isocortex, caudate putamen, and the hippocampal commisure. (e) volumetric rendering of T2* image, (f) smoothed rendering of 37 manually delimited structures, (g) 3-D rendering of WHS with gene expression correlation projection from hippocampus. Image credits: (a–d) Center for In Vivo Microscopy, Box 3302, Duke University Medical Center, Durham, North Carolina, United States of America; (e–g) Allen Institute for Brain Science, Seattle, Washington, United States of America.