A Computational and Experimental Study of the Regulatory Mechanisms of the Complement System
Figure 5
Model prediction and experimental verification of effects of C4BP under infection-inflammation condition.
(A–B) Simulation results. Predicted profiles of the deposited C3 after knocking down or over-expressing C4BP in the presence of PC (A) or GlcNAc (B). (C–D) Experimental data. Profiles of deposited C4BP (C) or C3 (D) across time points of 0–4 h under infection-inflammation condition via classical pathway (triggered by PC beads) or lectin pathway (triggered by GlcNAc beads) in untreated or treated sera with increased C4BP or decreased C4BP, were studied. The deposited protein was resolved in 12% reducing SDS PAGE and detected using polyclonal sheep anti-C4BP. Same amount of pure protein was loaded to each of the gels as the positive control (labeled as “C” in the image). The black triangles point to the peaks of the time serials data.