Sequence Motifs in MADS Transcription Factors Responsible for Specificity and Diversification of Protein-Protein Interaction
Figure 3
Effect of motif-based mutations on interaction patterns.
(A) Mutations were introduced based on predicted interaction motifs as explained in Figure 2. Different domains in MIKC MADS domain proteins are shown with colored boxes indicating the various regions in which point mutations were introduced. Below these, the various mutant MADS domain proteins that were generated are listed. The descriptions of the mutated proteins are colored based on the domain in which the mutation was generated. The mutated MADS domain proteins are SHORT VEGETATIVE PHASE (SVP1), AGAMOUS LIKE 24 (AGL24), SUPPRESSOR OF OVEREXPRESSION OF CO 1 (SOC1), APETALLA1 (AP1), CAULIFLOWER (CAL), and AGAMOUS (AG). Note that there are two double mutations for which one mutation occurs in the MADS/I domain and one in the K-box. Below each mutated protein, the number of losses and gains of protein-protein interactions in the yeast two-hybrid assay for the mutated proteins in comparison to the native MADS domain proteins is indicated (see Table 1 for interaction partner identities). (B) Histogram of F-scores for prediction of effect of mutants based on randomized input data (see text for details). The arrow indicates the F-score obtained by the predictor trained on experimental input data.