Simultaneous Genome-Wide Inference of Physical, Genetic, Regulatory, and Functional Pathway Components
Figure 4
Experimental validation of predicted synthetic lethal interactions.
Experimentally tested synthetic lethal hypotheses in the yeast A) DNA topological change and B) regulation of protein biosynthesis processes. A total of 20 gene pairs from our predicted synthetic lethality networks were experimentally tested using the SGA platform [4], [13]. We confirmed 14 of these interactions (70%), 8 in DNA topological change and 6 in protein biosynthesis. Several of the remaining unconfirmed pairs (e.g. GCS1 and SLT2; see main text) show additional evidence of condition-specific synthetic lethality.