A Computational Approach to Analyze the Mechanism of Action of the Kinase Inhibitor Bafetinib
Figure 3
Perturbed functional sub-network based on induction of apoptosis by intracellular signals.
In the protein-protein interaction (edges) network, the Bafetinib profile (grey nodes) perturbs the biological process (triangular nodes) which is pivotal in BCR-ABL dependent CML. The drug affinity (at) is indicated by the node size. Kinases in the target profile have a red label. Proteins of the uniform functional sub-network interacting with inhibited kinases are shown with a red node border. K562 cells contain ABL1 and its fusion protein BCR-ABL which is not found by the algorithm in this sub-network. However, ABL1 pulldown is hidden by BCR-ABL and hence missed as target. Western plots proved ABL1 as a competed target [33] and hence influences are indicated with thin red borders.