Endothelial Cell Capture of Heparin-Binding Growth Factors under Flow
Figure 10
Simulations predict binding affinity of FGF-2 for HSPG impacts FGF-2 capture more than affinity for FGFR.
(A) The affinity of FGF-2 for HSPG was varied in simulations by changing the association rate constant (•) or the dissociation rate constant (○). (B) The affinity of FGF-2 for FGFR was varied by changing the association rate constant (•) or the dissociation rate constant (○). The FGF-2 captured within the cell-lined hollow fiber (bound or internalized) at the given KD value after 5 min. was scaled by that same value from simulations using the base case KD value (Table 2). Arrow indicates base case KD.