Systems Biology of Coagulation Initiation: Kinetics of Thrombin Generation in Resting and Activated Human Blood
Figure 10
Simulating clotting times in whole blood.
The very small reaction volumes in a 384 well plate prevent us from studying coagulation reactions in whole blood (See text). To simulate the kinetics of initiation in whole blood we simulated clotting times for additions of TF, thrombin (IIa), IXa, Xa or combinations of all 3 proteases at low and high doses reported by Butenas et al. [49] in the Mann laboratory. We found good qualitative agreement between experimental clotting times (blue) and initiation times predicted by the Platelet-Plasma model (green). The Hockin-Mann (with fluorogenic thrombin substrate, SIIa) model (red) predicts finite initiation times only in the presence of high dose TF or Xa.