Systems Biology of Coagulation Initiation: Kinetics of Thrombin Generation in Resting and Activated Human Blood
Figure 9
Titration of TF and active proteases into blood.
(A) Effect of exogenous TF on initiation time. TF was titrated from 0.5 fM to 5 pM in 5× diluted blood. The black solid line is the simulated initiation time for the Hockin-Mann model (with SIIa) and the blue dashed line is the prediction of the Platelet-Plasma model. The light green solid line is the experimental control with no added TF. (B) Addition of prothrombinase components. Xa (red) and Va (green) was added to 5× diluted blood. The black solid line is the simulated initiation time for the Hockin-Mann model (with SIIa). The red and green dashed lines are the prediction of the Platelet-Plasma model for Xa and Va, respectively. The light green solid line is the experimental control with no added proteins. (C) Addition of VIIa, IXa and XIa. Various concentrations of VIIa (green), IXa (red) and XIa (blue) were added to blood at 5× dilution. The dashed lines of the corresponding color are simulations done with the Platelet-Plasma model. The light green solid line is the experimental control.