Systems Biology of Coagulation Initiation: Kinetics of Thrombin Generation in Resting and Activated Human Blood
Figure 8
Prevention of initiation on CVX activated platelets.
CTI-treated regular plasma or plasma deficient in factors VII, XI or XII were supplemented with washed (plasma free) platelets. These samples were left untreated or were treated with antibodies against TF; VII; XI; XII or XI, XII and VII simultaneously; and tested for thrombin generation without exogenous TF addition after activating platelets with 25 nM CVX. Simultaneous inhibition of XI, XII and VII activity was required to completely abolish thrombin generation.