Characterizing the Metabolism of Dehalococcoides with a Constraint-Based Model
Figure 5
Reconstructed cobalamin biosynthesis pathway of Dehalococcoides.
Dashed orange lines indicate cell membrane, grey lines indicate missing pathways, and red lines indicate existing pathways, putative genes of which are identified in Dehalococcoides during the reconstruction of iAI549. The arrows are denoting the directionality of the reactions. Since the genomes encode a putative cobalamin transporter, Dehalococcoides may salvage vitamin B12 either in the form of cobinamide or cobalamin from the environment as indicated by ‘cobinamide transport’ and ‘cobalamin transport’ reactions in the figure. The adenosylcobalamin, which is the end product of the entire pathway, is a biomass constituent and is assumed to take part in Dehalococcoides cell formation.