G-Quadruplex DNA Sequences Are Evolutionarily Conserved and Associated with Distinct Genomic Features in Saccharomyces cerevisiae
Figure 6
G4 DNA motifs form G4 DNA structures in vitro.
(A) Characterization of the five experimentally tested G4 DNA motifs. The motifs were selected to represent a range of genome locations and conditions. (B) Circular dichroism analysis of the three of the five G4 DNA motifs tested demonstrates that they form parallel G4 DNA structures. The G4 motif tested is indicated by the chromosome number above the graph. (C) Native acrylamide gel comparing the migration of oligonucleotides before and after the formation of the G4 DNA structure. The G4 DNA structure (indicated by the cartoon on the right) migrates more slowly than linear DNA. The G4 motif tested is indicated by the chromosome number beneath the gel.