Computer Simulation of Cellular Patterning Within the Drosophila Pupal Eye
Figure 2
Apical OC expansion and initial simulation inputs.
(A) TEM of an ommatidium in an 18 hour APF eye. The central photoreceptors (R), cone (c) and 1° cells are pseudo-colored green. Note that the left 1° nucleus (asterisks) is rising, that on the right (only partially seen) is already higher resulting in a larger apical profile; the neighboring 2° nuclei are basal, these cells have larger basal footprints. Select 2° cells are pseudo-colored orange. (B) The OC increases in apical surface area (µm2) over time as measured from live images. The error bars indicate one standard deviation. (C, D) Example of a 22 hour APF pupal eye stained with an E-cadherin-specific antibody to visualize surface cell boundaries. This image was traced to provide a starting point for the simulations, which were then run multiple times as noted. The region used in the tracing is false colored as in Figure 1. Bars represent 10 µm.