Plato's Cave Algorithm: Inferring Functional Signaling Networks from Early Gene Expression Shadows
Figure 6
JNK represses EGF-Stimulation of klf4.
A. Experimental results showing that JNK repression enhances EGF-stimulation of klf4. LβT2 cells were pre-incubated with SP600125 (JNK Inhibitor) for 30 minutes and treated with different concentration of EGF for 45 minutes. The levels of klf4 were measured by quantitative PCR and rps11 was used for normalization. EGF alone causes a slight induction of klf4, and inhibition of JNK results in a more significant induction. With concurrent JNK inhibition and EGF stimulation, a synergistic effect can be seen; B. The proposed biochemical interaction network involving JNK, EGF, EGFR, and klf4 that mediates the functional repression of EGFR by JNK.