Temporal Sensitivity of Protein Kinase A Activation in Late-Phase Long Term Potentiation
Figure 3
Experiments to verify that the critical inter-train interval for the PKA-dependence of L- LTP is ∼60 sec.
(A) LTP induced using a 80 sec inter-train interval (n = 7) is blocked by KT5720 (p<0.05), which demonstrates PKA dependence. (B) In contrast, when using a 40 sec inter-train interval (bottom; n = 7), LTP in KT5720 slices is not different from vehicle control (p>0.05), demonstrating PKA independence. In both panels, fEPSPs for KT5720 treated slices are shown with red circles, and vehicle controls with blue circles. (C) Summary of temporal sensitivity of PKA dependence of L-LTP. Data for 3 sec, 20 sec and 300 sec are from [3].