Stochastic Model of Integrin-Mediated Signaling and Adhesion Dynamics at the Leading Edges of Migrating Cells
Figure 2
Qualitative dependence of CHO.K1 cell motility on ECM density.
CHO.K1 cells expressing GFP-paxillin were filmed using TIRF microscopy as they migrated on the indicated densities of fibronectin ([FN], expressed as the coating concentration in µg/ml) for a period of 30 minutes. As the representative time courses show, only the intermediate concentration of 2 µg/ml supports a broad zone of persistent membrane protrusion. The images also show the monotonically positive dependence of stable adhesion abundance on [FN]. The illustrative plots show the predicted effects of myosin IIA (MIIA) depletion or overexpression.