Numerical Modelling Of The V-J Combinations Of The T Cell Receptor TRA/TRD Locus

doi:10.1371/journal.pcbi.1000682

Numerical Modelling Of The V-J Combinations Of The T Cell Receptor TRA/TRD Locus

Figure 2

Validation of the modelling approach: analysis of the V and J region uses.

(A) V region utilization: the X axis represents the V region in Kb. The Y axis shows the V gene percentage utilization in simulation. The simulated data sets have been normalized in order to be compared according to the following formula The fixed parameters of the simulation were as follow, one million of alpha chains, ongoing 1 to 4 rearrangements with opening speeds of 18 Kb/h and 1.03 Kb/h for the V and the J region respectively; (B) J region utilization: the X axis represents the J region in Kb; (C) and (D) Amplitude of J region utilization by opposite V genes, V1 (distal) and V21 (proximal). The X axis represents experimental quantification on 9 J genes. The Y axis shows the experimental utilization frequency of 9 J genes by the V1 and V21 genes. (E) and (F) Amplitude of J region utilization in the model. The X axis represents the J genes. The Y axis shows the model frequency utilization by each J genes. (G) and (H) Superposition of experimental and simulated data for the 9 J genes. The X axis represents experimental quantification on 9 J genes. V and J regions utilization from simulated data are similar to experimental data obtained from [9].

doi: https://doi.org/10.1371/journal.pcbi.1000682.g002