Identifying the Rules of Engagement Enabling Leukocyte Rolling, Activation, and Adhesion
Figure 6
In vivo and in silico results are compared.
In vivo conditions (from [13]): mice were either (A, B) WT or (C, D) KO. Red: wet-lab values; black: ISWBC2 values. Wet-lab experiments: post-capillary venules were observed from one minute before to one minute after CXCL1 injection at t = 0. Each simulation ran for 600 simulation cycles (equivalent to about 60 seconds). Individual leukocyte trajectories are plotted for (A) WT and (C) KO mice. (B, D) Individual leukocytes were tracked every 5 s, and those that were adherent during that time were counted. Open squares are adherent leukocyte counts for individual venules. Open circles are corresponding adherent leukocyte counts for venules. Thirty leukocytes comprised the population within a venule. Individual leukocytes were tracked every 50 simulation cycles (approximately 5 s), and those that were adherent were counted. ISWBC2: parameter values are those listed in Tables 4 and 5, and leukocytes are in the presence of cxcl1 beginning at t = 0. Lfa1 clustering was either (A, B) enabled or (C, D) disabled.