Dissecting Early Differentially Expressed Genes in a Mixture of Differentiating Embryonic Stem Cells
Figure 4
Enrichment of the RBP-J motif in the upstreams of the differentiation module.
(A) Average upstream binding affinity of RBP-J both shows enhanced signals in the upstream sequences of the differentiation module genes as compared to that of the pluripotency module genes. (B) Testing of all 332 non-redundant mammalian DNA binding motifs available in TRANSFAC v10.2, four motifs were found to be enriched in the upstream sequences of the differentiation module genes as compared to that of the pluripotency module genes (p-value ≤0.05). In particular, the RBP-J motif exhibited the second smallest p-value (0.028) and the largest enrichment factor (2.0) among the 332 motifs.