Introduction to the Quantitative Analysis of Two-Dimensional Fluorescence Microscopy Images for Cell-Based Screening
Figure 4
(A) Brightfield image of C. elegans worms not amenable to thresholding because of intensity variations. The color bar on the right of the image shows that brighter pixels are displayed as red and dimmer pixels as blue. Original image from the project described by Moy et al. [32]. (B) Contour plot of smooth illumination function fitted to one or a set of images such as (A). (C) Corrected image obtained by pixel-wise division of (A) by (B). (D) Worms in the corrected image are consistently darker than the background and can therefore be identified by thresholding.