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Alternative Splicing in the Differentiation of Human Embryonic Stem Cells into Cardiac Precursors

Figure 5

Genes with confirmed AS events have distinct domain-level changes.

RT-PCR results for a panel of predicted CP differentiation-splicing events with both a common CP-NP differentiation and CP-specific ANOVA pattern. Genes are categorized based on predicted domain/motif changes: truncation, disruption, modification, exchange or no associated predictions. The higher band in each gel image is the longer isoform with exon inclusion (in); the lower band is the shorter isoform with exon exclusion (ex), unless indicated as a constitutive (cs), mutually exclusive (mx), or miRNA (miR)-containing exon. Additional confirmed genes are shown in Figures 3 and 7 and are further described in Table 3.

Figure 5

doi: https://doi.org/10.1371/journal.pcbi.1000553.g005