The Mechanism of Ubiquitination in the Cullin-RING E3 Ligase Machinery: Conformational Control of Substrate Orientation
Figure 3
Comparison of snapshots from the simulations.
(A) Skp2; (B) Fbw7; (C) β-TrCP1; (D) Cdc4; (E) Fbs1; (F) TIR1; (G) pVHL; (H) SOCS2; (I) SOCS4. The unbound form trajectory 1(left), trajectory 2 (middle) and bound form (right) comparison is shown in each figure. Structural snapshots of the F-box, VHL-box and SOCS-box domains are superimposed. The snapshots are taken at 0 ns (orange for substrate binding proteins and blue for adaptor Skp1, ASK1 or Elongin C) and maximum rotation angle (green). The rotation angles of the substrate binding domain are shown.