Statistical Use of Argonaute Expression and RISC Assembly in microRNA Target Identification
Figure 4
System biology-compensated expression level comparisons of known target pairs.
Improvements in the percentages of variation in targeted mRNA expression are observed through use of the adjusted R2 statistic. Partial correlations of proxies to targeting Ago 2 RISCs and targeted mRNAs are lower than those of m/miRNA expressions. Paired Wilcoxon tests of the hypothesis H0: μ0−μ1 = 0 vs. HA: μ0−μ1≠0 were rejected for the Madison (p = 0.0135), Broad (p = 0.0186) and combined (p<0.0001) datasets, where μ0 and μ1 refer to mean correlations of marginal m/miRNA expression and mean partial correlations respectively.