A Novel Scoring Approach for Protein Co-Purification Data Reveals High Interaction Specificity
Figure 5
Indirect associations in the IDBOS-Gavin PIN and Y2H data sets are enriched with false negatives.
An indirect association occurs when two non-interacting proteins share an interaction partner, e.g., A and B represent an indirect association in the case of A–C–B. Indirect associations form a subset of all non-interactions. A false negative is defined as a non-interaction that is curated as a direct physical interaction in a reference set: (A) BGS, (B) SBMC2 (see Materials and Methods). The fraction of indirect associations that are false negatives (actual) was compared with the fraction of all non-interactions that are false negatives (expected). Enrichments were computed as ratios of actual/expected.