SpaK/SpaR Two-component System Characterized by a Structure-driven Domain-fusion Method and in Vitro Phosphorylation Studies
Figure 5
In vitro phosphorylation studies involving SpaK mutants.
A, B: Polyacrylamide gel electrophoresis of 6xHis-SpaR and 6xHis-SpaK wild type or mutants in isolation or in combination, in the presence of ATP. Lanes 1, 7: molecular weight markers. A: Coomassie blue staining. B: Autoradiography. Mutant1: H247Q, Mutant 2: G392A.