SpaK/SpaR Two-component System Characterized by a Structure-driven Domain-fusion Method and in Vitro Phosphorylation Studies
Figure 2
Homology model of the SpaR N-terminal (residues 1–117) and C-terminal (residues 118–220) domains.
Modeling of the N-terminal domain was based on PDB template 1mvo_A, and the C-terminal domain was based on PDB template 2gwr_A. The conformation between domains was modeled based on 2gwr (response regulator protein MTRA from Mycobacterium tuberculosis). Coloring scheme reflects consecutive ordering of amino acids from the N-terminal region (blue) to the C-terminal region (red). Residues in SpaR that correspond to the functional residues in response regulator 2ftk (Spo0F; see Table 2B) are displayed as sticks.