A Bipolar Clamp Mechanism for Activation of Jak-Family Protein Tyrosine Kinases
Figure 2
Critical analysis of the SH2-Bβ-mediated Jak2 autophosphorylation mechanism in vitro.
(A–C) Surface and contour plots of Jak2 autophosphorylation (Y2∼P) for varying concentrations and dimerization KD values of SH2-Bβ, and with three different KD values of Jak2/SH2-Bβ binding: (A) KD,JS = 100 nM; (B) KD,JS = 10 nM; (C) KD,JS = 1 nM. See text for a description of the model assumptions, following [14]. (D) Surface and contour plot of heterotetramer (JS2J) concentration for KD,JS = 1 nM.